Details

Rapid Cell Magnetisation Using Cationised Magnetoferritin


Rapid Cell Magnetisation Using Cationised Magnetoferritin


Springer Theses

von: Sara Correia Carreira

96,29 €

Verlag: Springer
Format: PDF
Veröffentl.: 21.06.2017
ISBN/EAN: 9783319603339
Sprache: englisch

Dieses eBook enthält ein Wasserzeichen.

Beschreibungen

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.<div>&nbsp;</div><div><br></div>
Introduction.- Materials and Methods.- Characterising Magnetoferritin and Cationised Magnetoferritin.- Stem Cell Labelling with Cationised Magnetoferritin.- Toxicological Profiling of Cationised Magnetoferritin.- Escherichia coli labelling with Cationised Magnetoferritin.- Overall Conclusions and Future Opportunities.
<div>Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.<br></div>
Nominated as an outstanding PhD thesis by the University of Bristol, Bristol, UK Presents a comprehensive introduction to magnetic nanoparticles and their use in magnetic cell labelling Includes a detailed protocol describing the synthesis and functionalisation of novel, protein-based magnetic nanoparticles for ultrafast and universal cell labelling Discusses in-depth the characterisation of magnetic labelling efficiency and the effects on stem cell biology Includes supplementary material: sn.pub/extras

Diese Produkte könnten Sie auch interessieren:

Proteases in the Brain
Proteases in the Brain
von: Uwe Lendeckel, Nigel M Hooper
PDF ebook
149,79 €
Alzheimer's Disease: Cellular and Molecular Aspects of Amyloid beta
Alzheimer's Disease: Cellular and Molecular Aspects of Amyloid beta
von: J. Robin Harris, Falk Fahrenholz
PDF ebook
213,99 €